دانلود رایگان پاورپوینت ارزیابی میزان سمی بودن پلی پلکس (انگلیسی)

An evaluation of in vitro and in vivo toxicity of chitosan-pDNA polyplexes
Gene delivery to lung
Cationic polymers:DNA complex
Bacterial plasmids
Objectives
Preparation of the chitosan/pDNA polyplex
Characterization
Gel Retardation Assay
Objectives
Cell lines
Cationic polymers:DNA complex
Transfection efficiency
Transfection experiment
Luciferase expression
MTS assay
Cytotoxicity assay: MTS
MTS assay (vary conc)
MTS assay
In vivo study
Luciferase expression in mice lungs
Number of cells in BAL fluids
Total protein and LDH activity
Cytokines
Conclusion
Future work

Gene delivery to lung
The transmission of DNA encoding for therapeutic gene or protein into the target cells for prevention or treatment of disease
Potential treatment for
Cystic fibrosis, Asthma
Advantages
Easily accessible via airways
Large surface area for transfection
Reduces the risk of systemic side effects
Disadvantages
Repulsion between DNA & cell membrane due to same charge
Enzymatic degradation